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| Registros recuperados: 116 | |
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| Reda,Fifi M.. |
| Abstract L-glutaminase was produced by Streptomyces canarius FR (KC460654) with an apparent molecular mass of 44 kDa. It has 17.9 purification fold with a final specific activity 132.2 U/mg proteins and 28% yield recovery. The purified L-glutaminase showed a maximal activity against L-glutamine when incubated at pH 8.0 at 40 °C for 30 min. It maintained its stability at wide range of pH from 5.0 11.0 and thermal stable up to 60 °C with Tm value 57.5 °C. It has high affinity and catalytic activity for L-glutamine (Km 0.129 mM, Vmax 2.02 U/mg/min), followed by L-asparagine and L-aspartic acid. In vivo, L-glutaminase showed no observed changes in liver; kidney functions; hematological parameters and slight effect on RBCs and level of platelets after 10 days... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: L-glutaminase; Anti-cancer; Cytotoxicity; MTT assay. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000400957 |
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| Silva,Mayara Christine; Matos,Aline Ferreira; Santos,Hataânderson Luíz Cabral dos; Gomes,Jennifer Vieira; Pastura,Danilo Giovanni Narciso; Pereira,Gabriela Lopes; Rocha,Ellen Borges da; Larangeira,Mayara de Jesus Costa; Alves,Raphael Santiago; Bastos,Leonardo de Oliveira; Borba,Helcio Resende; Lima,Viviane Moreira de. |
| Laurus nobilis L. is a large shrub belonging to the Lauracea family. Its leaves are widely used for food seasoning as well as in folk medicine. Various studies have demonstrated the antiproliferative, antifungal and antibacterial effects of Laurus nobilis, but no studies have investigated the genotoxic effect of the aqueous extract of the plant. The objective of this study was to analyze the genotoxic potential of an aqueous extract of leaves, using the Allium cepa assay and mouse peripheral blood cell micronucleus test. The results showed that the extract did not have any genotoxic activity, but cytotoxic activity was observed in the two experimental models used. The extract had an antiproliferative effect, detected through the reduction of the mitotic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Laurus nobilis; Allium cepa; Micronucleus; Genotoxicity; Cytotoxicity. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100534 |
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| Zanelatto,Leonardo Campos; Silva,Patrícia Benites Gonçalves da; Sartori,Daniele; Panis,Carolina; Lepri,Sandra; Fátima,Ângelo de; Mantovani,Mário Sérgio. |
| Abstract The identification of antitumoral substances is the focus of intense biomedical research. Two structural analogues of thalidomide, LNO3 and L3, are two synthetic compounds that might possess such antitumor properties. We evaluated the toxicological effects of these substances, including cytotoxicity, genotoxicity and induction of apoptosis in HTC cells. Additionally, the production of free radicals (nitric oxide and superoxide) was investigated, and the expression of caspases genes 3, 8, and 9 were determined by RT-qPCR. The compounds exhibited cytotoxic effects that resulted in inhibited cell proliferation. LNO3 showed to be more effective and toxic than L3 in all assays. LNO3 stimulated the release of NO and superoxide, which was accompanied by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Thalidomide analogues; Antiproliferative activity; Cytotoxicity; Free radicals; Apoptosis. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200270 |
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| Rolim,P.M.; Fidelis,G.P.; Padilha,C.E.A.; Santos,E.S.; Rocha,H.A.O.; Macedo,G.R.. |
| Melon (Cucumis melo L.) has high economic value and in recent years, its production has increased; however, part of the fruit is wasted. Usually, inedible parts such as peel and seeds are discarded during processing and consumption. Extracts of melon residues were prepared and their phenolic compounds, antioxidants and antiproliferative activities were evaluated. Total phenolic compounds were found in hydroethanolic, hydromethanolic, and aqueous extracts, especially for melon peel (1.016 mg gallic acid equivalent/100 g). Flavonoids total content found for melon peel aqueous extract was 262 µg of catechin equivalent (CA)/100 g. In all extracts of melon peel significant amounts of gallic acid, catechin, and eugenol were found. For total antioxidant capacity,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Melon residues; Antioxidant activity; Cancer cells; Free radical scavenging activity; Metal chelator; Cytotoxicity. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400607 |
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| Sadiq,Anwar Zeb Abdul; Ullah,Farhat; Ayaz,Sajjad Ahmad Muhammad. |
| BACKGROUND: Isodon rugosus is used traditionally in the management of hypertension, rheumatism, tooth-ache and pyrexia. Present study was arranged to investigate I. rugosus for phytoconstituents, phytotoxic and cytotoxic activities to explore its toxicological, pharmacological potentials and to rationalize its ethnomedicinal uses. Briefly, qualitative phytochemical analysis of the plant extracts were carried out for the existence of alkaloids, flavonoids, saponins, oils, glycosides, anthraquinones, terpenoids, sterols and tannins. Plant crude methanolic extract (Ir.Cr), its subsequent fractions; n-hexane (Ir.Hex), chloroform (Ir.Chf), ethyl acetate (Ir.EtAc), aqueous (Ir.Aq) and saponins (Ir.Sp) in different concentrations were tested for phytotoxic and... |
| Tipo: Journal article |
Palavras-chave: Isodon rugosus; Cytotoxicity; Phytotoxicity; Brine shrimps. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100051 |
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| Regalado, E.L.; Mendiola, J.; Laguna, A.; Nogueiras, C.; Thomas, O.P.. |
| A method involving flash chromatography, semi-preparative phenylhexyl RP HPLC-DAD-ELSD combined with analytic polar-RP HPLC-DAD, was applied to separate and purify six highly nitrogenated bases and a bicyclic amidine alkaloid, the major components of the marine sponge Niphates digitalis. Their structures were identified as 1,8-diazabicyclo[5.4.0]undec-7- ene (1), deoxycytidine (2), phenylalanine (3), adenosine (4), deoxyguanosine (5), adenine (6) and thymidine (7) on the basis of spectroscopic data analyses. This is the first report of these compounds in a marine sponge belonging to the Niphates genus and the first evidence of the presence of 1 from a natural source. |
| Tipo: Journal Contribution |
Palavras-chave: Sponges; Cytotoxicity; Cytotoxicity; Sponges; Http://aims.fao.org/aos/agrovoc/c_34251; Http://aims.fao.org/aos/agrovoc/c_7321. |
| Ano: 2010 |
URL: http://hdl.handle.net/1834/3860 |
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| Seoane, Marta; González-fernández, Carmen; Soudant, Philippe; Huvet, Arnaud; Esperanza, Marta; Cid, Ángeles; Paul-pont, Ika. |
| Due to the growing concern about the presence of microplastics (MP) in the environment, the number of studies evaluating the toxicity of these small persistent particles on different marine species has increased in recent years. Few studies have addressed their impact on marine phytoplankton, a subject of great concern since they are primary producers of the aquatic food web. The aim of this study is to unravel the cytotoxicity of 2.5 μg mL−1 unlabelled amino-modified polystyrene beads of different sizes (0.5 and 2 μm) on the marine diatom Chaetoceros neogracile. In addition to traditional growth and photosynthesis endpoints, several physiological and biochemical parameters were monitored every 24 h in C. neogracile cells by flow cytometry during their... |
| Tipo: Text |
Palavras-chave: Microalgae; Microplastic; Flow cytometry; Cytotoxicity; Scanning electron microscopy. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00492/60415/63850.pdf |
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| Faggio,Caterina; Morabito,Marina; Minicante,Simona Armeli; Piano,Giada Lo; Pagano,Maria; Genovese,Giuseppa. |
| Undaria pinnatifida (U. pinnatifida) is a highly invasive species and has caused concern all over the world because it has invaded coastal environments, has the potential to displace native species, significantly alters habitat for associated fauna, and disturbs navigation. Any attempt to eradicate it would be futile, owing to the elusive, microscopic gametophyte, and because the alga thrives in sites rich in anthropic activities. Venice Lagoon is the largest Mediterranean transitional environment and the spot of the highest introduction of non-indigenous species, including U. pinnatifida, which is removed as a waste. We demonstrated that polysaccharide extracts from U. pinnatifida have an anticoagulant effect on human blood in vitro and are not cytotoxic.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Algal extract; Anticoagulant activity; Cytotoxicity; Fucoidans; Human blood cells; Undaria pinnatifida. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500798 |
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| Carvalho Júnior,A.D.; Vieira,F.P.; De Melo,V.J.; Lopes,M.T.P.; Silveira,J.N.; Ramaldes,G.A.; Garnier-Suillerot,A.; Pereira-Maia,E.C.; De Oliveira,M.C.. |
| We encapsulated cisplatin into stealth pH-sensitive liposomes and studied their stability, cytotoxicity and accumulation in a human small-cell lung carcinoma cell line (GLC4) and its resistant subline (GLC4/CDDP). Since reduced cellular drug accumulation has been shown to be the main mechanism responsible for resistance in the GLC4/CDDP subline, we evaluated the ability of this new delivery system to improve cellular uptake. The liposomes were composed of dioleoylphosphatidylethanolamine (DOPE), cholesteryl hemisuccinate (CHEMS), and distearoylphosphatidylethanolamine-polyethyleneglycol 2000 (DSPE-PEG2000) and were characterized by determining the encapsulation percentage as a function of lipid concentration. Among the different formulations,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cisplatin; Liposomes; Encapsulation; Cytotoxicity. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800017 |
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| Silva, Erick Leal da; Sales, Ila Monize Sousa; Santos, Fabelina Karollyne Silva dos; Peron, Ana Paula. |
| The present study evaluated the acute toxicity at the cellular level of processed juice ready for consumption Orange and Grape flavors, produced by five companies with significant influence on the food market of South American countries, especially in Brazil. This evaluation was performed in root meristem cells of Allium cepa L., at the exposure times of 24 and 48 hours, directly with marketed liquid preparations. Based on the results, it was found that fruit juices, of all companies considered, promoted significant antiproliferative effect to root meristems at the exposure time of 24 hours and resulted in at both exposure times, statistically significant number of mitotic spindle changes and chromosomal breaks. Therefore, under the study conditions, all... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Industrialized juice; Cytotoxicity; Genotoxicity; Mutagenicity; Meristem tissue. |
| Ano: 2017 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/33699 |
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| Grisolia,Cesar K.; Rivero,Carla L. G.; Starling,Fernando L. R. M.; Silva,Izabel C. R. da; Barbosa,Antonio C.; Dorea,Jose G.. |
| Lake Paranoá is a tropical reservoir for the City of Brasilia, which became eutrophic due to inadequate sewage treatment associated with intensive population growth. At present, two wastewater treatment plants are capable of processing up to 95% of the domestic sewage, thereby successfully reducing eutrophization. We evaluated both genotoxic and cytotoxic parameters in several fish species ( Geophagus brasiliensis , Cichla temensis , Hoplias malabaricus , Astyanax bimaculatus lacustres , Oreochromis niloticus , Cyprinus carpio and Steindachnerina insculpita ) by using the micronucleus (MN) test, the comet assay and nuclear abnormality assessment in peripheral erythrocytes. The highest frequencies of MN were found in Cichla temensis and Hoplias... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotoxicity; Fish biomonitoring; Eutrophic lake; Micronucleus; Comet assay; Cytotoxicity. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100021 |
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| Araujo,Caroline de S.; Brito,Lorrane D.; Tarifa,Marina O.; Silva,Nayara J. Farah da; Rodrigues,Karoline S.; Cavalcante,Dalita G. S. M.; Gomes,Andressa S.; Zocoler,Marcos A.; Yoshihara,Eidi; Camparoto,Marjori L.; Job,Aldo E.; Kerche,Leandra E.. |
| Abstract This study evaluated the genotoxicity, mutagenicity, antigenotoxicity, and antimutagenicity effects on biochemical parameters of oxidative stress of the Spondias dulcis bark ethanolic extract on mice. The extract was evaluated in the doses of 500, 1000, and 1500 mg/kg bw via gavage. To evaluate the protective effects of the extract, benzo[a]pyrene (B[a]P) and cyclophosphamide (CP) were chosen as DNA damage inducers. Genotoxicity and antigenotoxicity were evaluated by the comet assay. Cytotoxicity, mutagenicity, and antimutagenicity were evaluated by the micronucleus test in bone marrow and peripheral blood. The biochemical parameters of oxidative stress were evaluated by the quantification of catalase activity (CAT) and reduced glutathione (GSH)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Comet assay; Micronucleus test; Spondias dulcis; Cytotoxicity; Mutagenicity. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400643 |
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| Choquet, Gwenaelle; Soudant, P; Lambert, C; Nicolas, Jean-louis; Paillard, C. |
| Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of other Vibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V tapetis compared to that of V splendidus and V pectenicida suggests a specific pathogenicity... |
| Tipo: Text |
Palavras-chave: Cytotoxicity; Flow cytometry; Vibrio tapetis; Ruditapes philippinarum; Bivalve hemocytes; Pathogenicity. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00000/10761/7590.pdf |
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| Giardia lamblia trophozoites were incubated for 2 h with activated murine macrophages, nitric oxide (NO) donors or a superoxide anion generator (20 mU/ml xanthine oxidase plus 1 mM xanthine). Activated macrophages were cytotoxic to Giardia trophozoites (~60% dead trophozoites). This effect was inhibited (>90%) by an NO synthase inhibitor (200 µM) and unaffected by superoxide dismutase (SOD, 300 U/ml). Giardia trophozoites were killed by the NO donors, S-nitroso-acetyl-penicillamine (SNAP) and sodium nitroprusside (SNP) in a dose-dependent manner (LD50 300 and 50 µM, respectively). A dual NO-superoxide anion donor, 3-morpholino-sydnonimine hydrochloride (SIN-1), did not have a killing effect in concentrations up to 1 mM. However, when SOD (300 U/ml)... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Nitric oxide; Macrophage; Peroxynitrite; Giardia lamblia; Cytotoxicity; Superoxide. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100015 |
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| BARROS,BÁRBARA R.S.; BARBOZA,BRUNO RAFAEL; RAMOS,BÁRBARA A.; MOURA,MAIARA C. DE; COELHO,LUANA C.B.B.; NAPOLEÃO,THIAGO HENRIQUE; CORREIA,MARIA TEREZA S.; PAIVA,PATRÍCIA MARIA G.; CRUZ FILHO,IRANILDO JOSÉ DA; SILVA,TÚLIO DIEGO DA; LIMA,CLÁUDIA S.A.; MELO,CRISTIANE M.L. DE. |
| Abstract: Currently, the research of new natural compounds with biological potential demonstrates great ethnopharmacological importance. In this study, we evaluated the biological properties promoted by saline extract from Malpighia emarginata DC leaves, whose objective is to evaluate the antioxidant, antimicrobial and cytotoxicity potential. Phytochemical characterization was performed by UPLC-MS chromatography to identify the chemical compounds. For the antioxidant potential, DPPH, ATT and FRAP methods were used. The antibacterial and antifungal tests were performed evaluating the MIC50, MIC90, CMB and CMF parameters. Moreover, antibiofilm action was evaluated. Cytotoxicity and proliferation were performed using splenocytes from Balb/c mice and were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioactive compounds; Cytotoxicity; Malpighia emarginata DC; Phytochemical. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100627 |
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| QUIGNARD,Etienne Louis Jacques; POHLIT,Adrian Martin; NUNOMURA,Sergio Massayoshi; PINTO,Ana Cristina da Silva; SANTOS,Elba Vieira Mustafa dos; MORAIS,Sabrina Kelly Reis de; ALECRIM,Alexandre Mascarenhas; PEDROSO,Andreza Cristiana da Silva; CYRINO,Barbara Rachel Brito; MELO,Christiane Santana de; FINNEY,Ellen Kathryn; GOMES,Erika de Oliveira; SOUZA,Katiuscia dos Santos de; OLIVEIRA,Laura Cristina Pereira de; DON,Luciana de Castro; SILVA,Luiz Francisco Rocha e; QUEIROZ,Maria Mireide Andrade; HENRIQUE,Marycleuma Campos; SANTOS,Mirian dos; PINTO,Patrícia de Souza; SILVA,Suniá Gomes. |
| 226 methanol and water extracts representing 74 mainly native plant species found in Amazonas State, Brazil, were tested at a standard concentration of 500 μg/mL for lethality towards larvae of the brine shrimp species Artemia franciscana. Several cytotoxic plant species were identified in this work: Aspidosperma marcgravianum, A. nitidum, Croton cajucara, Citrus limetta, Geissospermum argenteum, Minquartia guianensis, Piper aduncum, P. amapense, P. capitarianum, P. tuberculatum and Protium aracouchini. The results were analyzed within the context of the available traditional knowledge and uses for these plants. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Artemia franciscana; Cytotoxicity; Amazonian plants. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672003000100093 |
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| Registros recuperados: 116 | |
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